Journal: International Journal of Cardiology

Article Title: Cardiac inflammation and microvascular procoagulant changes are decreased in second wave compared to first wave deceased COVID-19 patients

doi: 10.1016/j.ijcard.2021.11.079

Figure Lengend Snippet: Comparison of coagulation regulating factors and CML in the cardiac microvasculature between first and second wave COVID-19 and control patients. The number of blood vessels positive for the procoagulant factors Tissue Factor (A; TF), Factor VII (B; FVII), Factor XII (C; FXII) and anticoagulant dipeptidyl peptidase 4 (D; DPP4) are shown per cm 2 of left ventricular heart tissue in control patients (Con; n = 18) and first wave (Wave 1; n = 15) and second wave (Wave 2; n = 10) COVID-19 patients. (E): An immunohistochemical example of the presence N(ε)-Carboxymethyllysine (CML) in the endothelium of intramyocardial blood vessels of a COVID-19 patient (arrow) and the immunohistochemical (IH) score for CML per cm 2 in control patients (Con) and first- and second wave COVID-19 patients. (F): The number of blood vessels with weak, moderate and strong CML staining (staining intensities 1, 2, 3 respectively) in control (Con) and first- and second wave COVID-19 patients. Each point in the graphs represents the value of one individual patient, the bars represent mean ± SD. The bars represent mean ± SD. For comparisons Kruskal-Wallis tests with Dunn's multiple comparison tests were used. *p < 0.05, **p < 0.01, *** p < 0.001, **** p < 0.0001 (exact p-values are given in the text).

Article Snippet: Primary antibodies were added for 1 h at room temperature (RT): mouse-anti-human CD45 (1:100, Dako Santa Clara, USA; M0701), rabbit-anti-human CD68 (1:400, Dako; M0814), rabbit-anti-human CD3 (1:100, Dako; A0452), rabbit-anti-human C3d (1:1000, Dako; A0063), mouse-anti-human CD31 (1:50, Dako; M0823), mouse-anti-human TF (1:250, Biorbyt Cambridge, UK; ORB100189), mouse-anti-human FVII (1:100, Sanquin Research, Amsterdam, The Netherlands), mouse-anti-human FXII (1:25, Sanquin), mouse-anti-human CML (1:500 [ ]) or mouse-anti-human CD26 (1:100, Bio-Rad, Lunteren, The Netherlands, MCA1317T).

Techniques: Comparison, Coagulation, Control, Immunohistochemical staining, Staining

Journal: Cell

Article Title: Spatial proteogenomics reveals distinct and evolutionarily conserved hepatic macrophage niches

doi: 10.1016/j.cell.2021.12.018

Figure Lengend Snippet: Combination of CITE-seq, scRNA-seq, snRNA-seq, and spatial analyses enables identification of all hepatic cell types including bona fide cell doublets, related to Figure 1 (A and B) Top DEGs (A) and DEPs (B) for cell types from Figure 1 B. (C) Distinct profiles of cells or nuclei within the UMAP depending on isolation protocols; 71,162 cells from ex vivo digestions, 96,066 cells from in vivo digestions, and 18,666 nuclei. Numbers on plots represent numbers of cells/nuclei per population. (D) Correlation plots showing genes captured within the KC, B cell and neutrophil populations with and without addition of CITE-seq antibodies. (E) Expression of VSIG4, CD206, and ESAM (protein, top) and Vsig4 , Mrc1 , and Esam (mRNA, bottom). (F) UMAP showing clusters of cells when only minimal QC for gene number and % mitochondrial genes is performed; 17,669 cells pooled from 3 samples. Expression of Cd5l, Cd19 , and Kdr by the clusters facilitating identification of cell types per annotation. (G) CITE-seq data from (F) in Flow-Jo showing expression of CD206 and ESAM in total KCs (left) and total B cells (middle). Numbers represent % of entire KC or B cell population. Identified populations were then mapped back onto the original UMAP (right). (H) Expression of CD31, CD26, and CD38 by indicated populations. (I) Heatmaps showing expression of top DEGs between KC1s and LSECs (left), KC2s and KC1s + LSECs (middle) and B cell2s and B cell1s + LSECs (right). (J) 3D reconstruction of murine liver following perfusion with antigen fix to inflate endothelial cells and staining with antibodies against CD31, CD206, and F4/80. (K) UMAP showing clusters generated from Visium analysis of liver tissue (4 samples) and liver capsule (1 sample). (L) Top unbiased genes defining zonation trajectory from portal to central vein in Visium. (M) Expression of Glul and Epcam by confocal microscopy (left), annotation of portal, periportal, mid, and central regions on same tissue section (middle) and overlay of both datasets (right). (N) Identification of cholangiocyte (left) and cDC (right) signatures on zonated Visium spots. (P) Molecular Cartography showing expression of indicated zonated hepatocyte mRNAs in liver tissue. Data are representative of 2 mice. (O) Expression of Itgae (encoding CD103) in the UMAP of the total liver (left) and flow cytometric analysis of total cDC1s for CD103 and MHCII expression in the healthy murine liver (right).

Article Snippet: Anti-Mouse CD26 (REA1196) PE , Miltenyi Biotec , 130-122-775; RRID: AB_2801934.

Techniques: Isolation, Ex Vivo, In Vivo, Expressing, Staining, Generated, Confocal Microscopy

Journal: Cell

Article Title: Spatial proteogenomics reveals distinct and evolutionarily conserved hepatic macrophage niches

doi: 10.1016/j.cell.2021.12.018

Figure Lengend Snippet:

Article Snippet: Anti-Mouse CD26 (REA1196) PE , Miltenyi Biotec , 130-122-775; RRID: AB_2801934.

Techniques: Purification, Recombinant, Staining, cDNA Synthesis, Gene Expression, Software, Microscopy

Journal: Immunity

Article Title: Airway Epithelial Cell-Derived Colony Stimulating Factor-1 Promotes Allergen Sensitization

doi: 10.1016/j.immuni.2018.06.009

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Anti-human CD26 , Fluidigm , BA5b, cat#3161015B.

Techniques: Antibody Labeling, Virus, Recombinant, Control, Enzyme-linked Immunosorbent Assay, Software

Journal: Hepatology (Baltimore, Md.)

Article Title: Fat‐associated lymphoid clusters as expandable niches for ectopic liver development

doi: 10.1002/hep.32277

Figure Lengend Snippet: List of antibodies used in this study

Article Snippet: DPPIV (CD26) , MCA2345A488 , Bio‐Rad, Hercules, CA , 1:50.

Techniques:

Journal: Drug Metabolism and Disposition

Article Title: Wuzhi Tablet ( Schisandra Sphenanthera Extract) Protects against Acetaminophen-Induced Hepatotoxicity by Inhibition of CYP-Mediated Bioactivation and Regulation of NRF2-ARE and p53/p21 Pathways

doi: 10.1124/dmd.114.059535

Figure Lengend Snippet: WZ possesses marked inhibitory effects on the activities of P450 enzymes and NAPQI-GSH formation. (A) Western blot analysis of CYP2E1, CYP1A2, CYP3A11, and glyceraldehyde-3-phosphate dehydrogenase levels in livers from control, WZ (700 mg/kg)-treated, APAP-treated, WZ (700 mg/kg)/APAP-treated mice. (B) Densitometric analysis of Western blots (n = 3). (C) Effect of WZ on the activities of CYP2E1, CYP1A2, CYP3A11 enzymes in mouse liver microsomes (n = 5). (D) Effect of WZ on NAPQI-GSH formation in mouse liver microsomes (n = 5). *P < 0.05, **P < 0.01, ***P < 0.001 versus control mice; #P < 0.05, ##P < 0.01 versus APAP-treated mice.

Article Snippet: CYP1A2 and CYP2E1 antibodies were purchased from Boster Biotechnology Co., Ltd. (Wuhang, China).

Techniques: Western Blot, Control